Rhizobium etli bv. mimosae, a novel biovar isolated from Mimosa affinis

Citation
Et. Wang et al., Rhizobium etli bv. mimosae, a novel biovar isolated from Mimosa affinis, INT J SY B, 49, 1999, pp. 1479-1491
Citations number
84
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
ISSN journal
0020-7713 → ACNP
Volume
49
Year of publication
1999
Part
4
Pages
1479 - 1491
Database
ISI
SICI code
0020-7713(199910)49:<1479:REBMAN>2.0.ZU;2-C
Abstract
Fifty rhizobial isolates from root nodules of Mimosa affinis, a small legum inous plant native to Mexico, were identified as Rhizobium etli on the basi s of the results of PCR-RFLP and RFLP analyses of small-subunit rRNA genes, multilocus enzyme electrophoresis and DNA-DNA homology. They are, however, a restricted group of lineages with low genetic diversity within the speci es. The isolates from M. affinis differed from the R. etli strains that ori ginated from bean plants (Phaseolus vulgaris) in the size and replicator re gion of the symbiotic plasmid and in symbiotic-plasmid-borne traits such as nifH gene sequence and organization, melanin production and host specifici ty. A new biovar. by. mimosae, is proposed within R. etli to encompass Rhiz obium isolates obtained from M. affinis. The strains from common bean plant s have been designated previously as R. etli by. phaseoli. Strains of both R. etli biovars could nodulate P. vulgaris, but only those of by. mimosae c ould form nitrogen-fixing nodules on Leucaena leucocephala.