Sporobacterium olearium gen, nov., sp, nov., a new methanethiol-producing bacterium that degrades aromatic compounds, isolated from an olive mill wastewater treatment digester

Citation
T. Mechichi et al., Sporobacterium olearium gen, nov., sp, nov., a new methanethiol-producing bacterium that degrades aromatic compounds, isolated from an olive mill wastewater treatment digester, INT J SY B, 49, 1999, pp. 1741-1748
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
ISSN journal
0020-7713 → ACNP
Volume
49
Year of publication
1999
Part
4
Pages
1741 - 1748
Database
ISI
SICI code
0020-7713(199910)49:<1741:SOGNSN>2.0.ZU;2-6
Abstract
A strictly chemo-organotrophic, anaerobic bacterium was isolated from an ol ive mill wastewater treatment digester on syringate and designated strain S R1(T). The cells were slightly curved rods, stained Gram-positive and posse ssed terminal spores. Strain SR1(T) utilized crotonate, methanol and a wide range of aromatic compounds including 3,4,5-trimethoxybenzoate (TMB), 3,4, 5-trimethoxycinnamate (TMC), syringate, 3,4,5-trimethoxyphenylacetate (TMPA ), 3,4,5-trimethoxyphenylpropionate (TMPP), ferulate, sinapate, vanillate, 3,4-dimethoxybenzoate, 2,3-dimethoxybenzoate, gallate, 2,4,6-trihydroxybenz oate (THB), pyrogallol, phloroglucinol and quercetin as carbon and energy s ources. Acetate and butyrate were produced from aromatic compounds, methano l and crotonate whereas methanethiol (MT) was produced from methoxylated ar omatic compounds and methanol. Strain SR1(T) had a G+C content of 38 mol% a nd grew optimally between 37 and 40 degrees C at ph 7.2 on a crotonate-cont aining medium. Phylogenetically, strain SR1(T) was a member of cluster XIVa of the Clostridiales group and shared a sequence similarity of 90% with Cl ostridium aminovalericum and Eubacterium fissicatena. Consequently, its pre cise neighbourliness to any one of them depended on the selection of strain s of the cluster. On the basis of the phylogenetic and phenotypic evidence presented in this paper, the designation of strain SR1(T) as Sporobacterium olearium gen, nov., sp. nov. is proposed. The type strain is SR1(T)(= DSM 12504(T)).