Two strains of a Brevibacterium-like bacterium originating from bumble-foot
lesions of domestic fowls were subjected to a polyphasic taxonomic study.
The phenotypic characteristics of the bacterium were consistent with its as
signment to the genus Brevibacterium although comparative 16S rRNA gene seq
uencing showed that the organism represents a distinct subline within the g
enus. Chromosomal DNA-DNA pairing studies confirmed that the unidentified b
acterium was genomically distinct and worthy of separate species status. Ba
sed on the phenotypic and genotypic distinctiveness of the bacterium from p
oultry, a new species, Brevibacterium avium, is proposed. The type strain o
f Brevibacterium avium is NCIMB 703055(T).