Mycobacterium wolinskyi sp nov and Mycobacterium goodii sp nov., two new rapidly growing species related to Mycobacterium smegmatis and associated with human wound infections: a cooperative study from the International Working Group on Mycobacterial Taxonomy

Citation
Ba. Brown et al., Mycobacterium wolinskyi sp nov and Mycobacterium goodii sp nov., two new rapidly growing species related to Mycobacterium smegmatis and associated with human wound infections: a cooperative study from the International Working Group on Mycobacterial Taxonomy, INT J SY B, 49, 1999, pp. 1493-1511
Citations number
56
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
ISSN journal
0020-7713 → ACNP
Volume
49
Year of publication
1999
Part
4
Pages
1493 - 1511
Database
ISI
SICI code
0020-7713(199910)49:<1493:MWSNAM>2.0.ZU;2-K
Abstract
Previous investigations demonstrated three taxonomic groups among 22 clinic al isolates of Mycobacterium smegmatis. These studies were expanded to 71 c linical isolates, of which 35 (49%) (group 1) were identical to five ATCC r eference strains including the type strain ATCC 19420(T). Twenty-eight isol ates (39%) were group 2, and eight isolates (11%) were group 3. Isolates of groups 2 and 3 were most often associated with post-traumatic or post-surg ical wound infections including osteomyelitis, were susceptible to sulfamet hoxazole, amikacin, imipenem and the tetracyclines, variably resistant to c larithromycin, and susceptible (group 1), intermediately resistant (group 2 ) or resistant (group 3) to tobramycin. The three groups were similar by ro utine biochemical and growth characteristics, but had different mycolic aci d dimethoxy-4-coumarinylmethyl ester elution patterns by HPLC and different PCR-restriction enzyme patterns of a 439 bp fragment of the hsp-65 gene. G roup 3 isolates differed from group 1 by 18 bp by 16S rRNA sequencing and e xhibited < 25% homology by DNA-DNA hybridization, being most closely relate d to Mycobacterium mageritense. The 16S rRNA of group 1 and group 2 isolate s differed by only 3 bp, but by DNA-DNA hybridization they exhibited only 4 0% homology. The following names are proposed: Mycobacterium goodii sp. nov . for group 2 isolates (type strain ATCC 700504(T) = MO69(T)), Mycobacteriu m wolinskyi sp. nov. for group 3 isolates (type strain ATCC 700010(T) = MO7 39(T)) and Mycobacterium smegmatis sensu stricto for group 1 isolates.