Two strictly aerobic, heterotrophic and mesophilic new organisms, strains K
itami A1 and Kitami C2(T), were isolated from the wastewater of a sugar-bee
t factory in Kitami City, Hokkaido, Japan. In batch cultures, these organis
ms produced both insoluble and soluble exopolysaccharides (EPSs) utilizing
sucrose as the sole carbon source. The G+C contents of the strains Kitami C
2(T) and Kitami A1 were 69.2 mol%, Both strains had anteiso-C15:0 acid, ant
eiso-C17:0 acid and iso-C16:0 as major components. The major isoprenoid qui
nones from these strains included menaquinone-11 and menaquinone-12. Physio
logical and biochemical characterization, phylogenetic analysis and DNA-DNA
relatedness indicated that these two organisms are new species of the genu
s Microbacterium, for which the name Microbacterium kitamiense is proposed.
The type strain of M kitamiense is strain Kitami C2(T) (= JCM 10270(T)).