M. Itoh et al., Spontaneous accumulation of eosinophils and macrophages throughout the stroma of the epididymis and vas deferens in alymphoplasia (aly) mutant mice: I. A histological study, AM J REPROD, 42(4), 1999, pp. 246-253
PROBLEM: The alymphoplasia (aly) mutation of mice causes the systemic absen
ce of lymph nodes and Peyer's patches. Histopathological analysis has revea
led that lymphocytes accumulate in some organs such as the salivary glands,
lungs, kidneys, liver, and pancreas of homozygotes (aly/aly) but not heter
ozygotes (aly/+). However, the presence of lymphocytic accumulation in male
reproductive tissues of aly/aly mice has not been reported.
METHOD OF STUDY: The male reproductive organs of homozygous aly/aly, hetero
zygous aly/+, and other immunodeficient mice bred in a specific pathogen-fr
ee state were histologically investigated.
RESULTS: Testes, ductuli efferentes, prostates, coagulating glands, and sem
inal vesicles of homozygous aly/aly mice were free from any pathological ac
cumulation of leukocytes. However, their epididymides and vasa deferentia e
xhibited massive infiltration by leukocytes, which were composed of eosinop
hils and macrophages. In all homozygotes examined, both eosinophils and mac
rophages were extensively observed throughout the stroma of the epididymis
and vas deferens, but never within the epithelium or lumen of the ducts, wi
th no parenchymal tissue damage. The accumulation of eosinophils was never
found in nonreproductive organs. In aly/+ heterozygous mice and other immun
odeficient mice, no pathological accumulation of leukocytes were noted.
CONCLUSIONS: The accumulation of eosinophils and macrophages is specific to
the epididymides and vasa deferentia of immunodeficient aly/aly homozygote
s, although the pathogenesis remains unknown.