K. Takakuwa et al., Possible susceptibility of the HLA-DPB1*0402 and HLA-DPB1*04 alleles to unexplained recurrent abortion: Analysis by means of polymerase chain reaction-restricted fragment length polymorphism method, AM J REPROD, 42(4), 1999, pp. 233-239
PROBLEM: To clarify whether HLA-DP antigens are associated with patient pop
ulation of unexplained recurrent abortion.
METHOD OF STUDY: The frequency of HLA-DPB1 alleles in patients with unexpla
ined recurrent abortion, and the compatibility of HLA-DPBI alleles between
patient couples, were studied using a polymerase chain reaction (PCR)-restr
icted fragment length polymorphism (RFLP) method. Thirty patients who had a
history of unexplained primary recurrent abortion, and their husbands, wer
e typed for HLA-DPB1 genotype. Two hundred and ninety-nine base pair fragme
nts from the second exon of HLA-DPB1 genes were selectively amplified using
the PCR-primers. After amplification, the DNAs were digested with restrict
ion endonucleases, and subjected to electrophoresis in a 12% polyacrilamide
gel to determine HLA-DPB1 genotype.
RESULTS: The frequency of HLA-DPB1*0402 and DPB1*04 alleles in the patient
group (n = 30) was significantly increased, as compared to that in the norm
al fertile women (n = 30). The frequency of HLA-DPB1*04 allele in the patie
nt group was significantly increased, as compared to that in the general po
pulation (n = 112). No significant compatibility of HLA-DPB1 alleles could
be observed between patient couples and normal fertile couples.
CONCLUSION: These findings suggest a possible new class II association with
patient population of unexplained recurrent abortion.