F4/80-positive cells rapidly accumulate around tubuli recti and rete testis between 3 and 4 weeks of age in the mouse: An immunohistochemical study

Citation
M. Itoh et al., F4/80-positive cells rapidly accumulate around tubuli recti and rete testis between 3 and 4 weeks of age in the mouse: An immunohistochemical study, AM J REPROD, 42(5), 1999, pp. 321-326
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
ISSN journal
1046-7408 → ACNP
Volume
42
Issue
5
Year of publication
1999
Pages
321 - 326
Database
ISI
SICI code
1046-7408(199911)42:5<321:FCRAAT>2.0.ZU;2-8
Abstract
PROBLEM: Previous studies demonstrated that F4/80 antigen (murine macrophag e-specific antigen)-positive cells in testes of normal adult mice accumulat e particularly in the interstitium adjacent to the tubuli recti and rete te stis (i.e., the central region). However, it remains unknown whether this a ccumulation is a congenital or acquired phenomenon. METHOD OF STUDY: The distribution of F4/80-positive cells on frozen section s of testes obtained from various aged mice was immunohistochemically exami ned to determine when the positive cells specifically accumulate in the cen tral region. RESULTS: F4/80-positive cells were homogeneously distributed throughout the testicular interstitium with no specific accumulation until 2 weeks of age . However, at 3 weeks of age, the density of positive cells in the central region became slightly, but significantly, higher than that in the intersti tium between the seminiferous tubules. Between 3 and 4 weeks of age, the ce ll density in the central region increased rapidly, the density at 4 weeks of age reaching the level of the mature testes of 8-week-old mice. CONCLUSION: These results demonstrate that the specific accumulation of F4/ 80-positive cells in the central region is an acquired phenomenon, which st arts and ends before puberty.