M. Itoh et al., F4/80-positive cells rapidly accumulate around tubuli recti and rete testis between 3 and 4 weeks of age in the mouse: An immunohistochemical study, AM J REPROD, 42(5), 1999, pp. 321-326
PROBLEM: Previous studies demonstrated that F4/80 antigen (murine macrophag
e-specific antigen)-positive cells in testes of normal adult mice accumulat
e particularly in the interstitium adjacent to the tubuli recti and rete te
stis (i.e., the central region). However, it remains unknown whether this a
ccumulation is a congenital or acquired phenomenon.
METHOD OF STUDY: The distribution of F4/80-positive cells on frozen section
s of testes obtained from various aged mice was immunohistochemically exami
ned to determine when the positive cells specifically accumulate in the cen
RESULTS: F4/80-positive cells were homogeneously distributed throughout the
testicular interstitium with no specific accumulation until 2 weeks of age
. However, at 3 weeks of age, the density of positive cells in the central
region became slightly, but significantly, higher than that in the intersti
tium between the seminiferous tubules. Between 3 and 4 weeks of age, the ce
ll density in the central region increased rapidly, the density at 4 weeks
of age reaching the level of the mature testes of 8-week-old mice.
CONCLUSION: These results demonstrate that the specific accumulation of F4/
80-positive cells in the central region is an acquired phenomenon, which st
arts and ends before puberty.