An inverse relation between the expression of tumor necrosis factor alpha (TNF-alpha) and TNF-alpha receptor in human endometrium

Citation
N. Chegini et al., An inverse relation between the expression of tumor necrosis factor alpha (TNF-alpha) and TNF-alpha receptor in human endometrium, AM J REPROD, 42(5), 1999, pp. 297-302
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
ISSN journal
1046-7408 → ACNP
Volume
42
Issue
5
Year of publication
1999
Pages
297 - 302
Database
ISI
SICI code
1046-7408(199911)42:5<297:AIRBTE>2.0.ZU;2-T
Abstract
PROBLEM: To determine whether the expression of tumor necrosis factor (TNF- alpha) correlates with TNF-alpha receptor expression in human endometrium. METHOD OF STUDY: A multiprimer synthetic cDNA standard template containing complimentary sequences for several cytokines including TNF-alpha and TNF-a lpha receptor type 2 was constructed and used in quantitative reverse trans cription polymerase chain reaction (Q-RT-PCR). RESULTS: Endometrium from proliferative phase of the menstrual cycle expres ses higher levels of TNF-alpha mRNA (2.35 +/- 0.2 x 10(5) copies/mu g of to tal cellular RNA) than secretory phase ([1.3 +/- 0.08 x 10(5) copies] P < 0 .05), with a significant reduction during menses (1.2 +/- 0.1 x 10(4) copie s) and postmenopausal period (8.1 +/- 1.6 x 10(4) copies [P < 0.051). In co ntrast, TNF-alpha type 1 receptor mRNA expression was higher in endometrium from the secretory phase (6.6 +/- 0.6 x 10(7) copies) compared to the mens es (5.1 +/- 0.5 x 10(6) copies), proliferative phase (1.9 +/- 0.1 x 10(6) c opies) and postmenopausal period (5.8 +/- 0.7 x 10(4) copies [P < 0.05]). C omparatively, TNF-alpha receptor type 2 is expressed 10 to 100 fold higher in the endometrium than TNF-alpha (P < 0.05). CONCLUSION: The data confirm that human endometrium expresses TNF-alpha; an d provide the first evidence that TNF-alpha expression is inversely related to TNF-alpha type 1 receptor expression during the menstrual cycle. Such a n inverse relation between TNF-alpha and TNF-alpha receptor expression may provide a regulatory mechanism necessary to overcome the detrimental effect of high levels of TNF-alpha on various endometrial cell types.