Novel hyperactive mitogen to endothelial cells: Human decidual NKG5

Citation
N. Langer et al., Novel hyperactive mitogen to endothelial cells: Human decidual NKG5, AM J REPROD, 42(5), 1999, pp. 263-272
Citations number
48
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
ISSN journal
1046-7408 → ACNP
Volume
42
Issue
5
Year of publication
1999
Pages
263 - 272
Database
ISI
SICI code
1046-7408(199911)42:5<263:NHMTEC>2.0.ZU;2-4
Abstract
PROBLEM: The purpose of this study was the isolation and characterization o f decidual extract proteins that exhibit mitogenicity on endothelial cells. METHOD OF STUDY: A partially purified extract (F-1 fraction) was obtained f rom human decidua of the first trimester of pregnancy. F-1 was separated by heparin-sepharose column and showed significant mitogenicity on bovine bra in capillary endothelial (BBCE) cells in vitro, using methylene blue stain nuclear assay. Sodium-dodecyl sulfate polyacrylamide gel electrophoresis (S DS-PAGE) revealed four fractions with MW less than 30 KDa. RESULTS: Mitogenic fraction, E11-12 (eluted at 1.5 M NaCl) was identified a s basic fibroblast growth factor (bFGF). Vascular endothelial growth factor (VEGF) and acidic (a)FGF were not identified as one of the mitogenic fract ions. However, fractions E5-6 and E7-8 induced statistically significant mi togenicity at concentration of 1 pg/mL, which is 10(3) times lower than bFG F. Sequencing of E5-6 fraction identified NKG5 protein, a putative secreted polypeptide derived from human natural killer (NK) cells and activated T c ells of unknown function. CONCLUSION: This work demonstrates that NKG5 stimulates mitogenicity of end othelial cells and may be involved in angiogenesis.