Release of bFGF, an endothelial cell survival factor, by osmotic shock

Citation
Me. Harnett et al., Release of bFGF, an endothelial cell survival factor, by osmotic shock, INV OPHTH V, 40(12), 1999, pp. 2945-2951
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
ISSN journal
0146-0404 → ACNP
Volume
40
Issue
12
Year of publication
1999
Pages
2945 - 2951
Database
ISI
SICI code
0146-0404(199911)40:12<2945:ROBAEC>2.0.ZU;2-H
Abstract
PURPOSE. To test the effects of osmotic change on basic fibroblast growth f actor (bFGF) release from cultured endothelial cells (ECs). METHODS. Bovine aortic and bovine retinal ECs were exposed to hypoosmotic s hock for 2 minutes, were allowed to recover for 15 minutes, and had bFGF re lease assayed. The role of bFGF in cell recovery was assessed by including neutralizing antibody against bFGF or the addition of exogenous bFGF, Cell number and viability were determined under varying conditions. Apoptosis wa s assessed by immunoperoxidase detection of digoxigenin-labeled DNA. RESULTS. After shock and recovery, both ECs released significantly greater amounts of bFGF than untreated control. bFGF release after shock fur 2 minu tes mas lower than release after shuck and recovery. Bovine retinal endothe lial (BRE) cell number was reduced at 48 hours after shock, recovery, and r emoval of released bFGF compared with cells left in the presence of release d bFGF, Cell number was significantly lower when BRE cells were shocked and recovered in the presence of a neutralizing anti-bFGF antibody (P < 0.05). Exogenous bFGF reversed this effect. Apoptosis was significantly increased in BRE cells shocked and recovered or in the presence of bFGF antibody (P < 0.001). CONCLUSIONS. bFGF is released by cultured ECs in response to osmotically in duced cell injury. These results support the concept of bFGF as a "wound" h ormone and survival factor for ECs. in further compromised tissue, release of bFGF in this manner mat play a role in the pathogenesis of disease.