Human gastrin: A Helicobacter pylori-specific growth factor

Citation
My. Chowers et al., Human gastrin: A Helicobacter pylori-specific growth factor, GASTROENTY, 117(5), 1999, pp. 1113-1118
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Gastroenerology and Hepatology","da verificare
Journal title
GASTROENTEROLOGY
ISSN journal
0016-5085 → ACNP
Volume
117
Issue
5
Year of publication
1999
Pages
1113 - 1118
Database
ISI
SICI code
0016-5085(199911)117:5<1113:HGAHPG>2.0.ZU;2-E
Abstract
Background & Aims: Helicobacter pylori resides within the gastric mucosa, a niche hostile to other microorganisms. Human gastrin levels are elevated a fter infection and return to normal after eradication. The aim of this stud y was to test the direct effect of gastrin on the growth of H. pylori. Meth ods: H. pylori and control bacteria were grown with gastrin or control pept ides and growth rate was determined. I-125-labeled gastrin was used to dete rmine uptake. Results: Human gastrin stimulated H. pylori growth in a speci fic, dose-dependent manner. Gastrin shortened the lag time, increased growt h rate in the logarithmic phase, and increased final bacterial concentratio n at the stationary phase. These effects were shown over a wide concentrati on range, including physiological luminal and serum levels. Labeled gastrin uptake was inhibited by unlabeled gastrin. Controls consisting of cholecys tokinin and pentagastrin inhibited gastrin uptake but did not stimulate gro wth. In contrast, somatostatin and epidermal growth factor had no effect on either gastrin uptake or bacterial growth. These results suggest a structu rally restricted, receptor-mediated, gastrin-specific effect. Conclusions: Human gastrin is a specific growth factor for H. pylori and may have a role in the adaptation of H. pylori to its unique habitat.