Elevated intracellular IFN-gamma levels in circulating CD8(+) lymphocytes in patients with fulminant hepatitis

Citation
K. Kimura et al., Elevated intracellular IFN-gamma levels in circulating CD8(+) lymphocytes in patients with fulminant hepatitis, J HEPATOL, 31(4), 1999, pp. 579-583
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Gastroenerology and Hepatology","da verificare
Journal title
JOURNAL OF HEPATOLOGY
ISSN journal
0168-8278 → ACNP
Volume
31
Issue
4
Year of publication
1999
Pages
579 - 583
Database
ISI
SICI code
0168-8278(199910)31:4<579:EIILIC>2.0.ZU;2-U
Abstract
Background/Aims: Fulminant hepatitis usually takes a rapidly progressive co urse, terminating in death within a short period. Experimental studies have demonstrated that immunological mechanisms play an important role, especia lly those involving virus-specific CD8(+) cytotoxic T lymphocytes and their production of interferon-gamma (IFN-gamma), However, there are no immunolo gical markers for prediction of the development of fulminant hepatitis in m an. Methods: Peripheral blood lymphocytes from four patients with fulminant hep atitis, six with acute hepatitis and 11 healthy volunteers as normal contro ls were analyzed, Intracellular IFN-gamma production in both CD8 positive a nd negative T lymphocytes was assessed by flow cytometry. Results: Populations of CD8(+) IFN-gamma(+) T lymphocytes were significantl y increased in patients with fulminant hepatitis, as compared with those wi th acute hepatitis and normal controls. Production of IFN-gamma in CD8(+) T lymphocytes of patients with fulminant hepatitis was also elevated, furthe rmore significantly correlating with the prothrombin time (r = -0.64, p<0.0 1), Conclusions: The capacity for IFN-gamma production by CD8(+) lymphocytes is up-regulated in fulminant hepatitis, and this may be important for the dev elopment of fulminant hepatitis.