Expansion of hematopoietic stem cells in vitro as a model system for humantissue engineering

Citation
Js. Greenberger et al., Expansion of hematopoietic stem cells in vitro as a model system for humantissue engineering, CLIN PLAST, 26(4), 1999, pp. 569
Citations number
59
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Surgery
Journal title
CLINICS IN PLASTIC SURGERY
ISSN journal
0094-1298 → ACNP
Volume
26
Issue
4
Year of publication
1999
Database
ISI
SICI code
0094-1298(199910)26:4<569:EOHSCI>2.0.ZU;2-8
Abstract
The authors have taken a new approach to finding optimal conditions for sti mulating conservative division of single isolated CD34+lin(-) hematopoietic stem cell candidates from human umbilical cord blood. The approach require d the design and development of a novel multi-well single cell combinatoria l culture system. This system incorporates the use of a multi-well tissue c ulture plate in which each well, can receive a single hematopoietic stem ce ll candidate. Sequential movement of each cell-containing well to a microsc opic imaging system, serially over a several-day to several-week experiment , is facilitated by computer control of a motorized stage and stabilization of the experiment in an environmentally controlled Bio-box built on the mi croscope stage. New image analysis software facilitates in the tracking of cell movement, recording of the time of fell division, and immunophenotypin g of each of multiple individual or recently doubled cells in real time by a robotically controlled pipetting station. The principles of single cell c ulture should help solve many problems in human hematopoietic stem cell exp ansion and also may be applicable to a wide range of other systems of inter est in tissue engineering.