In vivo genetic analysis of bacterial virulence

Citation
Sl. Chiang et al., In vivo genetic analysis of bacterial virulence, ANN R MICRO, 53, 1999, pp. 129-154
Citations number
72
Language
INGLESE
art.tipo
Review
Categorie Soggetti
Microbiology
Journal title
ANNUAL REVIEW OF MICROBIOLOGY
ISSN journal
0066-4227 → ACNP
Volume
53
Year of publication
1999
Pages
129 - 154
Database
ISI
SICI code
0066-4227(1999)53:<129:IVGAOB>2.0.ZU;2-C
Abstract
In vitro assays contribute greatly to our understanding of bacterial pathog enesis, but they frequently cannot replicate the complex environment encoun tered by pathogens during infection. The information gained from such studi es is therefore limited. In vivo models, on the other hand, can be difficul t to use, and this has to some extent diminished the incentive to perform s tudies in living animals. However, several recently developed techniques pe rmit in vivo examination of many genes simultaneously. Most of these method s fall into two broad classes: in vivo expression technology and signature- tagged mutagenesis. In vivo expression technology is a promoter-trap strate gy designed to identify genes whose expression is induced in a specific env ironment, typically that encountered in a host. Signature-tagged mutagenesi s uses comparative hybridization to isolate mutants unable to survive speci fied environmental conditions and has been used to identify genes critical for survival in the host. Both approaches have so far been used exclusively for investigating pathogen-host interactions, but they should be easily ad aptable to the study of other processes.