The luminol-amplified chemiluminescence of neutrophils and monocytes in patients with gastric cancer after intraoperative radiotherapy using radiosensitizer sanazole
I. Schepetkin et al., The luminol-amplified chemiluminescence of neutrophils and monocytes in patients with gastric cancer after intraoperative radiotherapy using radiosensitizer sanazole, CANC BIO R, 14(5), 1999, pp. 397-402
The effect of electron-beam intraoperntive radiotherapy (IORT) with radiose
nsitizer sanazole (drug AK-2123) on the functional activity, of neutrophils
and monocytes in patients with gastric cancer was studied with the use of
luminol-amplified chemiluminescence. Sanazole was administered intravenousl
y in a dose of 1.2 g/m(2), 30 min before IORT. After resection of the tumor
a single dose of 10 Gy was given to the field of regional lymph collectors
and the bed of the removed tumor applying a small-size betatron MIB-6E. Ce
lls were separated and chemiluminescence was estimated on the I-st day befo
re and on the 3-d clay and 2-d week after IORT with sanazole. Insignificant
effect on the zymosan- or phorbol-12-myristare-13-acetate (PMA)-stimulated
chemiluminescence was observed in this group, although there was found a c
onsiderable increase of chemiluminescence of neutrophils in response to zym
osan and PMA in 5 from 8 patients, and chemiluminescence of monocytes - in
3 from 8 patients. Correlation between individual indices of zymosan- and P
MA-stimulated chemiluminescence of neutrophils before IORT with sanazole wa
s not high, r=0.71, bur it increased significantly to r=0.91 on the 3-d day
and to r=0.96 on 2-d week after treatment. Correlation between individual
indices of stimulated chemiluminescence of monocytes before IORT with sanaz
ole was r=0.90, and increased to r=0.99 on the 3-d day and to r=0.96 on the
2-d week after treatment So, it is indicative of a correcting effect of th
e combined treatment including IORT with sanazole administration on the fun
ctional activity of neutrophils and monocytes, and the absence of functiona
l disturbances of these cells.