The luminol-amplified chemiluminescence of neutrophils and monocytes in patients with gastric cancer after intraoperative radiotherapy using radiosensitizer sanazole

Citation
I. Schepetkin et al., The luminol-amplified chemiluminescence of neutrophils and monocytes in patients with gastric cancer after intraoperative radiotherapy using radiosensitizer sanazole, CANC BIO R, 14(5), 1999, pp. 397-402
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology,"Onconogenesis & Cancer Research
Journal title
CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
ISSN journal
1084-9785 → ACNP
Volume
14
Issue
5
Year of publication
1999
Pages
397 - 402
Database
ISI
SICI code
1084-9785(199910)14:5<397:TLCONA>2.0.ZU;2-8
Abstract
The effect of electron-beam intraoperntive radiotherapy (IORT) with radiose nsitizer sanazole (drug AK-2123) on the functional activity, of neutrophils and monocytes in patients with gastric cancer was studied with the use of luminol-amplified chemiluminescence. Sanazole was administered intravenousl y in a dose of 1.2 g/m(2), 30 min before IORT. After resection of the tumor a single dose of 10 Gy was given to the field of regional lymph collectors and the bed of the removed tumor applying a small-size betatron MIB-6E. Ce lls were separated and chemiluminescence was estimated on the I-st day befo re and on the 3-d clay and 2-d week after IORT with sanazole. Insignificant effect on the zymosan- or phorbol-12-myristare-13-acetate (PMA)-stimulated chemiluminescence was observed in this group, although there was found a c onsiderable increase of chemiluminescence of neutrophils in response to zym osan and PMA in 5 from 8 patients, and chemiluminescence of monocytes - in 3 from 8 patients. Correlation between individual indices of zymosan- and P MA-stimulated chemiluminescence of neutrophils before IORT with sanazole wa s not high, r=0.71, bur it increased significantly to r=0.91 on the 3-d day and to r=0.96 on 2-d week after treatment. Correlation between individual indices of stimulated chemiluminescence of monocytes before IORT with sanaz ole was r=0.90, and increased to r=0.99 on the 3-d day and to r=0.96 on the 2-d week after treatment So, it is indicative of a correcting effect of th e combined treatment including IORT with sanazole administration on the fun ctional activity of neutrophils and monocytes, and the absence of functiona l disturbances of these cells.