Expression of co-stimulatory 4-1BB ligand induces significant tumor regression and protective immunity

Authors
Citation
J. Xiang, Expression of co-stimulatory 4-1BB ligand induces significant tumor regression and protective immunity, CANC BIO R, 14(5), 1999, pp. 353-361
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology,"Onconogenesis & Cancer Research
Journal title
CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
ISSN journal
1084-9785 → ACNP
Volume
14
Issue
5
Year of publication
1999
Pages
353 - 361
Database
ISI
SICI code
1084-9785(199910)14:5<353:EOC4LI>2.0.ZU;2-V
Abstract
Co-stimulatory molecules play an important role in initiating antitumor imm une responses. Engineered tumor cells expressing co-stimulatory molecules h ave been used as cancer vaccines in both experimental tumor models and clin ical trials In this study, we cloned a cDNA gene coding for the mouse co-st imulatory molecule 4-1BBL by RTPCR. The expression vector pCI-4-1BBL was co nstructed by DNA recombinant technology and further transfected into a mode rately immunogenic EL4 and a poorly immunogenic BL6-10 tumor cell line. Exp ression of the co-stimulatory molecule 4-1BBL is able to induce tumor regre ssion of EL4/4-1BBL but not BL6-10/4-1BBL tumor cell line in syngeneic BALB /c mice. The tumor regression which is mainly mediated by CD8(+) T cells fu rther leads to protective immunity against the parental EL4 tumor. Our resu lts thus indicate the potential utility of engineered tumor cells expressin g co-stimulatory molecule 4-1BBL, especially in combination with other co-s timulatory molecules such as B7-1 in cancer vaccine.