Comparisons of alloreactive potential of clinical hematopoietic grafts

Citation
W. Leung et al., Comparisons of alloreactive potential of clinical hematopoietic grafts, TRANSPLANT, 68(5), 1999, pp. 628-635
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
TRANSPLANTATION
ISSN journal
0041-1337 → ACNP
Volume
68
Issue
5
Year of publication
1999
Pages
628 - 635
Database
ISI
SICI code
0041-1337(19990915)68:5<628:COAPOC>2.0.ZU;2-J
Abstract
Background. We sought to compare the immunoreactive potential of human cord blood (CB) versus normal adult bone marrow (BM) versus mobilized blood (pe ripheral blood stem cells; PBSC) from cancer patients. Methods. Forty mice were randomized to receive a range of doses of T cell-r eplete cell preparations from one of the above three cell. sources. Twenty- eight control mice underwent transplantation with T cell-depleted cells. Mi ce were observed for 60 days for the development of fatal xenogeneic graft- versus-host disease-like syndrome (GVHDLS). Results. For the mice that had received T cell-replete grafts of CB or BM o r PBSC, the duration of GVHDLS-free survival of the chimeras was inversely proportional to the number of T cells transplanted. After adjustment for th e number of T cells transplanted, the relative hazard of developing fatal G VHDLS was 62-fold higher for PBSC and 210-fold higher for BM as compared wi th CB. Flow cytometric and histologic analyses of selected chimeras that di ed of GVHDLS showed extensive proliferation of human T cells in multiple or gans. In contrast, mice that survived to day 60 were engrafted with human m yeloid and B lymphoid cells. Conclusions. The immunoreactive potential, as measured by this in vivo assa y, differed among clinical grafts: BM > PBSC > CB.