Proviral DNA in the brains of goats infected with caprine arthritis-encephalitis virus

Citation
E. Sanna et al., Proviral DNA in the brains of goats infected with caprine arthritis-encephalitis virus, J COMP PATH, 121(3), 1999, pp. 271-276
Citations number
8
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Veterinary Medicine/Animal Health","Medical Research Diagnosis & Treatment
Journal title
JOURNAL OF COMPARATIVE PATHOLOGY
ISSN journal
0021-9975 → ACNP
Volume
121
Issue
3
Year of publication
1999
Pages
271 - 276
Database
ISI
SICI code
0021-9975(199910)121:3<271:PDITBO>2.0.ZU;2-Y
Abstract
Archive paraffin wax-embedded sections of brain from goats and kids natural ly infected with caprine arthritis-encephalitis virus (CAEV) were examined. Severe leucoencephalitis was present, with infiltration of lymphocytes, pl asma cells and macrophages into the while matter, meninges and choroid plex us. On both CAEV-positive and negative (control) tissues, in-situ polymeras e chain reactions were used to amplify a DNA sequence specific to the provi ral Pol region. In the infected tissues, strong hybridization signals were observed, mainly located in macrophages, microglial cells, astrocytes and o ligodendrocytes, and in the ependymal epithelium and choroid plexus. Positi ve areas were also found in the spinal cord in endothelial cells of small b lood vessels. Some neurons showed a positive reaction. (C) 1999 Harcourt Pu blishers Ltd.