Diversity in butane monooxygenases among butane-grown bacteria

Citation
N. Hamamura et al., Diversity in butane monooxygenases among butane-grown bacteria, APPL ENVIR, 65(10), 1999, pp. 4586-4593
Citations number
51
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
0099-2240 → ACNP
Volume
65
Issue
10
Year of publication
1999
Pages
4586 - 4593
Database
ISI
SICI code
0099-2240(199910)65:10<4586:DIBMAB>2.0.ZU;2-H
Abstract
Butane monooxygenases of butane-grown Pseudomonas butanovora, Mycobacterium vaccae JOB5, and an environmental isolate, CF8, were compared at the physi ological level. The presence of butane monooxygenases in these bacteria was indicated by the following results. (i) O-2 was required for butane degrad ation. (ii) 1-Butanol was produced during butane degradation. (iii) Acetyle ne inhibited both butane oxidation and 1-butanol production. The responses to the known monooxygenase inactivator, ethylene, and inhibitor, allyl thio urea (ATU), discriminated butane degradation among the three bacteria. Ethy lene irreversibly inactivated butane oxidation by P. butanovora but not by RI. vaccae or CF8. In contrast, butane oxidation by only CF8 was strongly i nhibited by ATU. In all three strains of butane-grown bacteria, specific po lypeptides were labeled in the presence of [C-14]acetylene. The [C-14]acety lene labeling patterns were different among the three bacteria. Exposure of lactate-grown CFS and P. butanovora a and glucose-grown M. vaccae to butan e induced butane oxidation activity as well as the specific acetylene-bindi ng polypeptides. Ammonia was oxidized by all three bacteria. P. butanovora oxidized ammonia to hydroxylamine, while CF8 and M. vaccae produced nitrite . All three bacteria oxidized ethylene to ethylene oxide. Methane oxidation was not detected by any of the bacteria. The results indicate the presence of three distinct butane monooxygenases in butane-grown. P. butanovora, M. vaccae, and CF8.