The effect of omeprazole dosing on the isolation of Helicobacter pylori from gastric aspirates

Citation
Mp. Williams et al., The effect of omeprazole dosing on the isolation of Helicobacter pylori from gastric aspirates, ALIM PHARM, 13(9), 1999, pp. 1161-1169
Citations number
44
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology,"da verificare
Journal title
ALIMENTARY PHARMACOLOGY & THERAPEUTICS
ISSN journal
0269-2813 → ACNP
Volume
13
Issue
9
Year of publication
1999
Pages
1161 - 1169
Database
ISI
SICI code
0269-2813(199909)13:9<1161:TEOODO>2.0.ZU;2-#
Abstract
Background: Animal experiments suggest that omeprazole dosing increases she dding of Helicobacter into the gastric lumen, and hence into gastric juice. Aim: To assess the effect of omeprazole dosing on the yield of H. pylori fr om gastric aspirates of infected volunteers, Methods: Six serial nasogastric aspirates, three before and three during do sing with omeprazole 40 mg b.d., were obtained for culture from 10 H, pylor i infected volunteers and one uninfected volunteer. To reduce contamination , samples were diluted 1:10 with Maximum Recovery Diluent (MRD: pH 7.0) or HCl-KCl buffer (pH 2.2) prior to culture on Columbia and Dent's agar, Results: Undiluted gastric juice cultures were rapidly overgrown by upper r espiratory tract flora, HCl-KCl dilution resulted in isolation of H, pylori from 77% of infected subject aspirates before, and 67% of aspirates during dosing with omeprazole, The yields were significantly lower with MRD dilut ion, 47% and 10%, respectively, Omeprazole dosing significantly decreased t he yield after MRD dilution, but not after HCl-KCl dilution. Conclusions: Decreasing intragastric acidity, by dosing with omeprazole, de creases the isolation of H. pylori from routinely processed gastric aspirat es. In vitro acidification of gastric aspirates, by HCl-KCl dilution, incre ases the isolation of H. pylori both before and during omeprazole dosing.