5 '-Hydroxycotinine-N-oxide, a new nicotine metabolite isolated from rat urine

Citation
G. Schepers et al., 5 '-Hydroxycotinine-N-oxide, a new nicotine metabolite isolated from rat urine, XENOBIOTICA, 29(8), 1999, pp. 793-801
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
XENOBIOTICA
ISSN journal
0049-8254 → ACNP
Volume
29
Issue
8
Year of publication
1999
Pages
793 - 801
Database
ISI
SICI code
0049-8254(199908)29:8<793:5'ANNM>2.0.ZU;2-1
Abstract
1. 5'-Hydroxycotinine-N-oxide, 5-(3-pyridyl-N-oxide)-5-hydroxy-1-methyl-pyr rolidone-2, was identified as a new in vivo metabolite of nicotine. 2. The new metabolite was isolated from the urine of rats treated with S-ni cotine and characterized using chemical and spectrometric methods. 3. 5'-Hydroxycotinine-N-oxide was synthesized and characterized by MS and b y infrared as well as H-1- and C-13-NMR spectroscopy. 4. Identity of the new metabolite with synthetic 5'-hydroxycotinine-N-oxide was demonstrated by comparing the MS and H-1-NMR spectroscopy data as well as by cochromatography of a spiked urine sample.