Production and characterization of anti-Kell monoclonal antibodies using transfected cells as the immunogen

Citation
Tht. Chu et al., Production and characterization of anti-Kell monoclonal antibodies using transfected cells as the immunogen, BR J HAEM, 106(3), 1999, pp. 817-823
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Hematology,"Cardiovascular & Hematology Research
Journal title
BRITISH JOURNAL OF HAEMATOLOGY
ISSN journal
0007-1048 → ACNP
Volume
106
Issue
3
Year of publication
1999
Pages
817 - 823
Database
ISI
SICI code
0007-1048(199909)106:3<817:PACOAM>2.0.ZU;2-R
Abstract
Monoclonal antibodies (Mabs) to blood group antigens are valuable as diagno stic reagents for typing red blood cells (RBCs) in the clinical setting, an d for structure-function studies of proteins, Here, we report a powerful sy stem that enabled us to produce Mabs to blood group antigens. A murine eryt hroleukaemia (MEL) cell line expressing Kell protein, a transmembrane glyco protein that carries a number of clinically relevant antigens, was used as a novel immunogen. Mabs with different specificities to the Kell protein we re produced from a single mouse fusion: an anti-js(b) (MIMA-8), and two ant ibodies (MIMA-9 and MIMA-10) with novel specificities, that reacted with RB Cs with the common Kell phenotype but not with RBCs with K+k- or Kp(a+b-) o r K-0 phenotypes, The non-reactivity with both K+k- or Kp(a+b-) RBCs implie d that the epitope was influenced by the molecular changes associated with an absence of the it or Kp(b) antigens. MIMA-8 is the first example of a Ma b anti-js(b) and was used in the clinical laboratory for screening donor RB Cs for Js(b-) blood and for typing RBCs from patients even when the RBCs we re coated with anti-IgG as is the case in autoimmune haemolytic anaemia. He avy and light chain variable regions of MIMA-8 were cloned and the sequence is given, This study illustrates the potential of this novel immunization approach for malting monoclonal antibodies to blood group antigens.