cDNA cloning, expression and chromosomal localization of the mouse mitochondrial thioredoxin reductase gene

Citation
A. Miranda-vizuete et al., cDNA cloning, expression and chromosomal localization of the mouse mitochondrial thioredoxin reductase gene, BBA-GENE ST, 1447(1), 1999, pp. 113-118
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
ISSN journal
0167-4781 → ACNP
Volume
1447
Issue
1
Year of publication
1999
Pages
113 - 118
Database
ISI
SICI code
0167-4781(19991006)1447:1<113:CCEACL>2.0.ZU;2-3
Abstract
Cytosolic thioredoxin (Trx) and thioredoxin reductase (TrxR) comprise a ubi quitous system that uses the reducing power of NADPH to act as a general di sulfide reductase system as well as a potent antioxidant system. Human and rat mitochondria contain a complete thioredoxin system different from the o ne present in the cytosol. The mitochondrial system is involved in the oxid ative stress protection through a mitochondrial thioredoxin-dependent perox idase. We report here the cDNA cloning and chromosomal localization of the mouse mitochondrial thioredoxin reductase gene (TrxR2). The mouse TrxR2 cDN A encodes for a putative protein of 527 amino acid residues with a calculat ed molecular mass of 57 kDa, that displays high homology with the human and rat counterparts. The N-terminus of the protein displays typical features of a mitochondrial targeting sequence with absence of acidic residues and a bundance of basic residues. Mouse TrxR2 also contains a stop codon in frame at the C-terminus of the protein, necessary for the incorporation of selen ocysteine that is required for enzymatic activity. The typical stem-loop st ructure (SECIS element) that drives the incorporation of selenocysteine is identified in the 3'-UTR. Northern analysis of the mouse TrxR2 mRNA shows a similar pattern of expression with the human homologue, with higher expres sion in liver, heart and kidney. Finally, we have assigned the mouse TrxR2 gene to chromosome 16 mapping at 11.2 cM from the centromer and linked to t he catechol-o-methyltransferase (comt) gene. (C) 1999 Elsevier Science B.V. All rights reserved.