Quantification of the mycotoxin patulin by a stable isotope dilution assay

Citation
M. Rychlik et P. Schieberle, Quantification of the mycotoxin patulin by a stable isotope dilution assay, J AGR FOOD, 47(9), 1999, pp. 3749-3755
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Agricultural Chemistry","Chemistry & Analysis
Journal title
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN journal
0021-8561 → ACNP
Volume
47
Issue
9
Year of publication
1999
Pages
3749 - 3755
Database
ISI
SICI code
0021-8561(199909)47:9<3749:QOTMPB>2.0.ZU;2-Y
Abstract
Two stable isotope dilution assays for the quantification of patulin [4-hyd roxy-4H-furo[3,2-c]pyran-2(6H)-one] in! foods were developed using C-13-lab eled patulin as the internal standard. One method was performed by means of LC/MS in negative electrospray ionization mode without derivatization; the other used HRGC/HRMS after trimethylsilylation of the patulin isotopomers. In comparison with previously reported methods based on high-performance l iquid chromatography with UV detection, HRGC/HRMS of the derivatized sample s showed better repeatability, higher recovery rates (96% at a spike level of 200 ng/L), and a 100 times lower detection limit (12 ng/L). In contrast, LC/MS showed a much lower performance as compared to HPLC/UV or HRGC/HRMS. Using HRGC/HRMS, the mycotoxin was quantified in many different fruit prod ucts and in molded wheat bread.