Two stable isotope dilution assays for the quantification of patulin [4-hyd
roxy-4H-furo[3,2-c]pyran-2(6H)-one] in! foods were developed using C-13-lab
eled patulin as the internal standard. One method was performed by means of
LC/MS in negative electrospray ionization mode without derivatization; the
other used HRGC/HRMS after trimethylsilylation of the patulin isotopomers.
In comparison with previously reported methods based on high-performance l
iquid chromatography with UV detection, HRGC/HRMS of the derivatized sample
s showed better repeatability, higher recovery rates (96% at a spike level
of 200 ng/L), and a 100 times lower detection limit (12 ng/L). In contrast,
LC/MS showed a much lower performance as compared to HPLC/UV or HRGC/HRMS.
Using HRGC/HRMS, the mycotoxin was quantified in many different fruit prod
ucts and in molded wheat bread.