N. Andersson et al., Gastrin effects on isolated rat enterochromaffin-like cells following long-term hypergastrinaemia in vivo, BBA-MOL CEL, 1451(2-3), 1999, pp. 297-304
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
The enterochromaffin-like (ECL) cells Flay an important role in the regulat
ion of gastric acid secretion. They respond to gastrin by a prompt increase
in histamine secretion, an effect which is mediated by the CCK-(B)/gastrin
receptor acting through the IP3/DAG pathway. In the rat, long-term treatme
nt with acid secretion inhibitors induces hypergastrinaemia which, in turn,
results in ECL cell hypertrophy and hyperplasia. The aim of the present st
udy was to evaluate various functional parameters in acutely isolated rat E
CL cells, following long-term hypergastrinaemia in vivo. Rats were treated
with vehicle or a supramaximal daily dose of omeprazole for more than 10 we
eks to ensure ECL cell hyperplasia. ECL cells were isolated from vehicle-tr
eated animals and 24, 72 and 120 h after the last dose of omeprazole. The f
unctional activity of the acutely isolated ECL cells was determined by meas
uring gastrin-and forskolin-induced histamine secretion. Changes in cytosol
ic free calcium upon gastrin stimulation were monitored by digital video im
aging. ECL cells successively regained their ability to respond to gastrin
following long-term hypergastrinaemia, reaching close to vehicle-treated le
vels 120 h after the last dose of omeprazole. In the rat, the response patt
ern of the ECL cells appears to normalise in parallel with the normalisatio
n of plasma gastrin levels. (C) 1999 Elsevier Science B.V. All rights reser
ved.