Gastrin effects on isolated rat enterochromaffin-like cells following long-term hypergastrinaemia in vivo

Citation
N. Andersson et al., Gastrin effects on isolated rat enterochromaffin-like cells following long-term hypergastrinaemia in vivo, BBA-MOL CEL, 1451(2-3), 1999, pp. 297-304
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
ISSN journal
0167-4889 → ACNP
Volume
1451
Issue
2-3
Year of publication
1999
Pages
297 - 304
Database
ISI
SICI code
0167-4889(19990921)1451:2-3<297:GEOIRE>2.0.ZU;2-R
Abstract
The enterochromaffin-like (ECL) cells Flay an important role in the regulat ion of gastric acid secretion. They respond to gastrin by a prompt increase in histamine secretion, an effect which is mediated by the CCK-(B)/gastrin receptor acting through the IP3/DAG pathway. In the rat, long-term treatme nt with acid secretion inhibitors induces hypergastrinaemia which, in turn, results in ECL cell hypertrophy and hyperplasia. The aim of the present st udy was to evaluate various functional parameters in acutely isolated rat E CL cells, following long-term hypergastrinaemia in vivo. Rats were treated with vehicle or a supramaximal daily dose of omeprazole for more than 10 we eks to ensure ECL cell hyperplasia. ECL cells were isolated from vehicle-tr eated animals and 24, 72 and 120 h after the last dose of omeprazole. The f unctional activity of the acutely isolated ECL cells was determined by meas uring gastrin-and forskolin-induced histamine secretion. Changes in cytosol ic free calcium upon gastrin stimulation were monitored by digital video im aging. ECL cells successively regained their ability to respond to gastrin following long-term hypergastrinaemia, reaching close to vehicle-treated le vels 120 h after the last dose of omeprazole. In the rat, the response patt ern of the ECL cells appears to normalise in parallel with the normalisatio n of plasma gastrin levels. (C) 1999 Elsevier Science B.V. All rights reser ved.