Medium optimization for efficient somatic embryogenesis and plant regeneration from immature inflorescences and immature scutella of elite cultivars of wheat, barley and tritordeum

Citation
F. Barro et al., Medium optimization for efficient somatic embryogenesis and plant regeneration from immature inflorescences and immature scutella of elite cultivars of wheat, barley and tritordeum, EUPHYTICA, 108(3), 1999, pp. 161-167
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences
Journal title
EUPHYTICA
ISSN journal
0014-2336 → ACNP
Volume
108
Issue
3
Year of publication
1999
Pages
161 - 167
Database
ISI
SICI code
0014-2336(1999)108:3<161:MOFESE>2.0.ZU;2-N
Abstract
Media have been developed for somatic embryogenesis and plant regeneration from immature inflorescences and immature scutella of elite cultivars of wh eat, barley and tritordeum. For wheat and tritordeum inflorescences, regene ration from embryogenic calluses induced on medium with picloram was almost twice as efficient as regeneration from cultures induced on 2,4-dichloroph enoxyacetic acid (2,4-D). The addition of zeatin at 5 or 10 mg l(-1) to reg eneration media had a positive effect on regeneration. For scutella, the hi ghest frequencies of embryogenesis (85%) and regeneration (50%) was obtaine d using an induction medium containing 2 mg l(-1) of 2,4-D and half concent ration of aminoacids. The morphogenetic capacities of 19 different cultivar s of wheat, barley and tritordeum were compared, and clear differences were found both between explants and genotypes. In wheat, embryogenic capacity from inflorescences (average of 92%) was higher than from immature scutella (average of 62%). However, shoot regeneration from scutella was clearly hi gher than from inflorescences (averages of 63%, and 18% respectively). Freq uencies of regeneration in wheat and barley varied widely among the cultiva rs tested and in both species no difference was found between spring and wi nter varieties.