Medium optimization for efficient somatic embryogenesis and plant regeneration from immature inflorescences and immature scutella of elite cultivars of wheat, barley and tritordeum
F. Barro et al., Medium optimization for efficient somatic embryogenesis and plant regeneration from immature inflorescences and immature scutella of elite cultivars of wheat, barley and tritordeum, EUPHYTICA, 108(3), 1999, pp. 161-167
Media have been developed for somatic embryogenesis and plant regeneration
from immature inflorescences and immature scutella of elite cultivars of wh
eat, barley and tritordeum. For wheat and tritordeum inflorescences, regene
ration from embryogenic calluses induced on medium with picloram was almost
twice as efficient as regeneration from cultures induced on 2,4-dichloroph
enoxyacetic acid (2,4-D). The addition of zeatin at 5 or 10 mg l(-1) to reg
eneration media had a positive effect on regeneration. For scutella, the hi
ghest frequencies of embryogenesis (85%) and regeneration (50%) was obtaine
d using an induction medium containing 2 mg l(-1) of 2,4-D and half concent
ration of aminoacids. The morphogenetic capacities of 19 different cultivar
s of wheat, barley and tritordeum were compared, and clear differences were
found both between explants and genotypes. In wheat, embryogenic capacity
from inflorescences (average of 92%) was higher than from immature scutella
(average of 62%). However, shoot regeneration from scutella was clearly hi
gher than from inflorescences (averages of 63%, and 18% respectively). Freq
uencies of regeneration in wheat and barley varied widely among the cultiva
rs tested and in both species no difference was found between spring and wi
nter varieties.