ELISA for IgG-class antibody to hepatitis E virus based on a highly conserved, conformational epitope expressed in Eschericia coli

Citation
Da. Anderson et al., ELISA for IgG-class antibody to hepatitis E virus based on a highly conserved, conformational epitope expressed in Eschericia coli, J VIROL MET, 81(1-2), 1999, pp. 131-142
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF VIROLOGICAL METHODS
ISSN journal
0166-0934 → ACNP
Volume
81
Issue
1-2
Year of publication
1999
Pages
131 - 142
Database
ISI
SICI code
0166-0934(199908)81:1-2<131:EFIATH>2.0.ZU;2-1
Abstract
In assays based on most recombinant hepatitis E virus (HEV) antigens, the I gG antibody responses to HEV are observed commonly to wane or disappear aft er the acute phase of infection. Such IgG assays have therefore been used f or the diagnosis of acute HEV infection, but they have limited usefulness i n seroepidemiological studies. Using western immunoblotting, it was shown p reviously that the open reading frame (ORF) 2.1 antigen, representing the c arboxy-terminal 267 amino acids (aa) of the capsid protein, exposes a confo rmational epitope which allows optimal detection of convalescent antibody c ompared to other proteins expressed in Escherichia coli. This conformationa l epitope is shown to be highly conserved between divergent human HEV isola tes, and the development of a sensitive and highly specific enzyme immunoas say (ELISA) based on this recombinant antigen is described. The ORF2.1 ELIS A allows the detection and quantitation of both acute- and convalescent pha se HEV-specific IgG, and will help to define better the antibody responses to the virus and the prevalence of HEV infection worldwide. (C) 1999 Elsevi er Science B.V. All rights reserved.