Comparison of five anaerobic incubation methods for enumeration of Clostridium perfringens from foods

Citation
A. Riley et al., Comparison of five anaerobic incubation methods for enumeration of Clostridium perfringens from foods, J FOOD PROT, 62(9), 1999, pp. 1041-1044
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Food Science/Nutrition
Journal title
JOURNAL OF FOOD PROTECTION
ISSN journal
0362-028X → ACNP
Volume
62
Issue
9
Year of publication
1999
Pages
1041 - 1044
Database
ISI
SICI code
0362-028X(199909)62:9<1041:COFAIM>2.0.ZU;2-9
Abstract
This study compared the cost, speed, convenience, and sensitivity of five a naerobic systems. Fung's double-tube (FDT) method, the minitube method (MT) , the sandwiched microtiter plate (SMP) method, and the Mitsubishi AnaeroPa ck System were compared with the Brewer anaerobic jar for total anaerobic b acterial counts in foods. Incubation was at 37 degrees C for 4, 8, 12, and 24 h. The results indicated that FDT, MT, SMP, and the Mitsubishi AnaeroPac k System were as sensitive as the Brewer anaerobic jar for the detection an d enumeration of Clostridium perfringens from food products. The FDT, MT, a nd SMP methods recovered higher numbers of C. perfringens compared to the B rewer anaerobic jar (P < 0.05) after 12 and 24 h incubation. The Brewer ana erobic jar was the most expensive method.