Malignant transformation of NIH3T3 cells by overexpression of early lymphocyte activation antigen CD98

Citation
K. Hara et al., Malignant transformation of NIH3T3 cells by overexpression of early lymphocyte activation antigen CD98, BIOC BIOP R, 262(3), 1999, pp. 720-725
Citations number
42
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006-291X → ACNP
Volume
262
Issue
3
Year of publication
1999
Pages
720 - 725
Database
ISI
SICI code
0006-291X(19990907)262:3<720:MTONCB>2.0.ZU;2-R
Abstract
CD98, which forms a heterodimer of relative molecular mass (M-r) 125, 000, was originally identified as an early T cell activation antigen. It consist s of a heavy chain of M-r 85,000 that bears the CD98 epitope and a light ch ain of M-r 40, 000. CD98 is strongly expressed on the surface of activated lymphocytes and various tumor cells irrespective of tissue origins. To inve stigate the participation of CD98 in cellular proliferation and malignant t ransformation, we established and characterized human CD98-transfected NIH3 T3 clones. Although the doubling times of the control cells and CD98-transf ected clones were almost the same, CD98-transfected clones grew to a higher saturation density than control cells. Efficiency of colony formation in s oft agar was augmented in CD98-transfected clones, and this augmentation wa s significantly reduced by antihuman CD98 mAb. Furthermore, CD98-transfecte d clones developed tumors in athymic mice. These results indicated that ove rexpression of CD98 participates in the process of malignant transformation , suggesting that CD98 has oncogenic potential. (C) 1999 Academic Press.