Characterization of fmtA, a gene that modulates the expression of methicillin resistance in Staphylococcus aureus

Citation
H. Komatsuzawa et al., Characterization of fmtA, a gene that modulates the expression of methicillin resistance in Staphylococcus aureus, ANTIM AG CH, 43(9), 1999, pp. 2121-2125
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
ISSN journal
0066-4804 → ACNP
Volume
43
Issue
9
Year of publication
1999
Pages
2121 - 2125
Database
ISI
SICI code
0066-4804(199909)43:9<2121:COFAGT>2.0.ZU;2-Q
Abstract
FmtA is a factor which affects the methicillin resistance level in methicil lin-resistant Staphylococcus aureus, Since FmtA has two of three conserved; ed motifs which are typically found in penicillin-binding proteins (PBPs) a nd p-lactamases, we investigated the penicillin-binding activity of recombi nant FmtA and found no such activity, Immunoblotting analysis revealed that FmtA localizes in the membrane fraction. To investigate the function of Fm tA, high-pressure liquid chromatography analysis of cell wall muropeptides was performed with an fmtA-inactivated mutant and its parent. The mutant sh owed a reduced cross-linking and partially reduced amidation of glutamate r esidues in the peptidoglycan of the mutant. The transcription of fmtA was d ose dependently increased by the addition of p-lactam antibiotics, fosfomyc in, and bacitracin, while its transcription was not changed by the addition of vancomycin or tetracycline. These results reveal that Fmt is a membrane -located, non-penicillin-binding protein and that mutation of fmtA affects the cell wall structure, although its precise function is still unknown.