Effect of radiation on the xanthine oxidoreductase system in the liver of mice

Citation
M. Srivastava et Rk. Kale, Effect of radiation on the xanthine oxidoreductase system in the liver of mice, RADIAT RES, 152(3), 1999, pp. 257-264
Citations number
48
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Experimental Biology
Journal title
RADIATION RESEARCH
ISSN journal
0033-7587 → ACNP
Volume
152
Issue
3
Year of publication
1999
Pages
257 - 264
Database
ISI
SICI code
0033-7587(199909)152:3<257:EOROTX>2.0.ZU;2-X
Abstract
The xanthine oxidoreductase system is one of the major sources of free radi cals in many pathophysiological conditions. Since ionizing radiations cause cell damage and death, the xanthine oxidoreductase system may contribute t o the detrimental effects in irradiated systems. Therefore, modulation of t he xanthine oxidoreductase system by radiation has been examined in the pre sent study. Female Swiss albino mice (7-8 weeks old) were irradiated with g amma rays (1-9 Gy) at a dose rate of 0.023 Gy s (-1) and the specific activ ities of xanthine oxidase (XO) and xanthine dehydrogenase (XDH) were determ ined in the liver of the animals. The mode and magnitude of change in the s pecific activities of XO and XDH were found to depend on radiation dose, At doses above 3 Gy, the specific activity of XO increased rapidly and contin ued to increase with increasing dose, Howe ver, the specific activity of XD H was decreased. These findings are suggestive of an inverse relationship b etween the acl:livity of XO and XDH. The ratio of the activity of XDH to th at of XO decreased with radiation dose. However, the total activity (XDH XO) remained constant at all doses, These results indicate that XDH may be converted into XO, An intermediate form, D/O, appears to be transient in th e process of conversion. The enhanced specific activity of XO may cause oxi dative stress that contributes to the radiation damage and its persistence in the postirradiation period, Radiation-induced peroxidative damage determ ined in terms of the formation of TEARS and the change in the specific acti vity of lactate dehydrogenase support this possibility. (C) 1999 by Radiati on Research Society.