Cloning and characterization of human and mouse PROSC (proline synthetase co-transcribed) genes

Citation
S. Ikegawa et al., Cloning and characterization of human and mouse PROSC (proline synthetase co-transcribed) genes, J HUM GENET, 44(5), 1999, pp. 337-342
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
JOURNAL OF HUMAN GENETICS
ISSN journal
1434-5161 → ACNP
Volume
44
Issue
5
Year of publication
1999
Pages
337 - 342
Database
ISI
SICI code
1434-5161(1999)44:5<337:CACOHA>2.0.ZU;2-L
Abstract
Large-scale DNA sequencing, coupled with in silico gene trapping, is a robu st approach to identifying unknown genes in selected genomic regions. Using this approach we have isolated a novel human gene, PROSC (for proline synt hetase co-transcribed [bacterial homolog]), from human chromosome 8p11.2, a nd its mouse counterpart. The human PROSC gene spanned 17kb of genomic DNA; its cDNA was 2530bp long, with 8 exons that included an open reading frame of 825 bp (275 amino acids). The mouse cDNA (Prosc), 1995 bp long, was pre dicted to encode 274 amino acids. PROSC is ubiquitously expressed in human tissues and has been highly conserved among divergent species from bacteria to mammals, suggesting its important cellular function. The gene product i s likely to be a soluble cytoplasmic protein, but its function remains to b e determined.