Stimulation of haematogenous liver metastases by ischaemia-reperfusion in rats

Citation
Ys. Ku et al., Stimulation of haematogenous liver metastases by ischaemia-reperfusion in rats, EURO J SURG, 165(8), 1999, pp. 801-807
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Surgery
Journal title
EUROPEAN JOURNAL OF SURGERY
ISSN journal
1102-4151 → ACNP
Volume
165
Issue
8
Year of publication
1999
Pages
801 - 807
Database
ISI
SICI code
1102-4151(199908)165:8<801:SOHLMB>2.0.ZU;2-A
Abstract
Objective: To find out whether hepatic ischaemia-reperfusion stimulates hep atic tumour metastases using a cell line of rat ascitic hepatoma (AH130). Design: Prospective experimental study. S etting: University laboratories, Japan. Materials: 118 male Donryu rats. Intervention: After laparotomy alone (group 1, n=35) or laparotomy and 20-m inutes ischaemia (group 2, n=34) or laparotomy and 30-minutes ischaemia (gr oup 3, n=34) of the median and left hepatic lobes, the animals were given e ither an intraportal injection of 1 x 10(5) or an intravenous injection of 1 x 10(6) viable AH130 cells. Main outcome measures: 10 days after inoculation of tumour cells the number of nodules on the surface of the right lobe and of the median plus left lo bes were separately counted for each Liver. Results: Irrespective of the route of tumour inoculation in group 1, there was no significant difference in the number of tumours/ g liver between the right and the median plus left lobes. However, in groups 2 and 3, the numb er of tumours/g liver in the median plus left lobes was significantly highe r than in the right lobe (p < 0.05). Furthermore, in the median plus left l obes, animals who had had 30 minutes of ischaemia had significantly more tu mours than those in the other two groups (p < 0.01). Conclusion: Hepatic ischaemia-reperfusion may increase the risk of developm ent of haematogenous liver metastases, by stimulating tumour cell-endotheli al cell interactions.