L. Chayet et al., HUMAN PLACENTAL ECTOENZYMES - STUDIES ON THE PLASMA-MEMBRANE ANCHORAGE AND EFFECT OF INHIBITORS OF ATP-METABOLIZING ENZYMES, Research communications in molecular pathology and pharmacology, 96(1), 1997, pp. 14-24
The human placental microvillar membrane contains several ectoenzymes,
including 5'-nucleotidase, alkaline phosphatase and ATP-diphosphohydr
olase (ATPD-PH), which might be involved in the extracellular metaboli
sm of nucleotides. The type of anchorage to the plasma membrane of the
two first enzymes has been shown to be via a glycosyl-phosphatidylino
sitol. In the present study, using an enzymatic approach, we show that
the ATP-DPH should be attached to the plasma membrane through a diffe
rent type of anchorage. We were also interested in the search of compo
unds which could interact differentially with this enzyme to be used a
s a tool for studying the other two hydrolytic enzymes in the presence
of ATP-DPH. Here we report several inhibitors of ecto-ATPases which s
eem to be a useful tool for studying these three enzymes.