HUMAN PLACENTAL ECTOENZYMES - STUDIES ON THE PLASMA-MEMBRANE ANCHORAGE AND EFFECT OF INHIBITORS OF ATP-METABOLIZING ENZYMES

Citation
L. Chayet et al., HUMAN PLACENTAL ECTOENZYMES - STUDIES ON THE PLASMA-MEMBRANE ANCHORAGE AND EFFECT OF INHIBITORS OF ATP-METABOLIZING ENZYMES, Research communications in molecular pathology and pharmacology, 96(1), 1997, pp. 14-24
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Pharmacy",Pathology,Biology
ISSN journal
1078-0297
Volume
96
Issue
1
Year of publication
1997
Pages
14 - 24
Database
ISI
SICI code
1078-0297(1997)96:1<14:HPE-SO>2.0.ZU;2-5
Abstract
The human placental microvillar membrane contains several ectoenzymes, including 5'-nucleotidase, alkaline phosphatase and ATP-diphosphohydr olase (ATPD-PH), which might be involved in the extracellular metaboli sm of nucleotides. The type of anchorage to the plasma membrane of the two first enzymes has been shown to be via a glycosyl-phosphatidylino sitol. In the present study, using an enzymatic approach, we show that the ATP-DPH should be attached to the plasma membrane through a diffe rent type of anchorage. We were also interested in the search of compo unds which could interact differentially with this enzyme to be used a s a tool for studying the other two hydrolytic enzymes in the presence of ATP-DPH. Here we report several inhibitors of ecto-ATPases which s eem to be a useful tool for studying these three enzymes.