Removal of detergent and solvent from solvent-detergent-treated immunoglobulins

Citation
A. Trescec et al., Removal of detergent and solvent from solvent-detergent-treated immunoglobulins, J CHROMAT A, 852(1), 1999, pp. 87-91
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF CHROMATOGRAPHY A
ISSN journal
0021-9673 → ACNP
Volume
852
Issue
1
Year of publication
1999
Pages
87 - 91
Database
ISI
SICI code
0021-9673(19990806)852:1<87:RODASF>2.0.ZU;2-G
Abstract
The solvent-detergent (S/D) method was applied for inactivation of lipid-en veloped viruses during the production of immunoglobulins. Amberlite XAD-7 r esin was used for removal of solvent (tri-n-butyl phosphate, TnBP) and dete rgent (Triton X-100) after the performed S/D inactivation procedure. The S/ D reagents from the immunoglobulin preparation were adsorbed on Amberlite X AD-7, while immunoglobulins passed through the column and retained their bi ological activity. Using the method developed here, the final immunoglobuli n preparation contains less than 1 ppm of Triton X-100 and less than 2 ppm TnBP. (C) 1999 Elsevier Science B.V. All rights reserved.