Contribution of Salmonella typhimurium virulence factors to diarrheal disease in calves

Citation
Rm. Tsolis et al., Contribution of Salmonella typhimurium virulence factors to diarrheal disease in calves, INFEC IMMUN, 67(9), 1999, pp. 4879-4885
Citations number
53
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
INFECTION AND IMMUNITY
ISSN journal
0019-9567 → ACNP
Volume
67
Issue
9
Year of publication
1999
Pages
4879 - 4885
Database
ISI
SICI code
0019-9567(199909)67:9<4879:COSTVF>2.0.ZU;2-8
Abstract
Limited knowledge is available about the virulence mechanisms responsible f or diarrheal disease caused by Salmonella typhimurium. To assess the contri bution to diarrheal disease of virulence determinants identified in models of infection, we tested a collection of S. typhimurium mutants for their ab ility to cause enteritis in calves. S. typhimurium strains carrying mutatio ns in the virulence plasmid (spvR), Salmonella pathogenicity island 2 (SPI- 2) (spiB), or SPI-5 (sopB) caused mortality and acute diarrhea in calves. A n S. typhimurium rfaJ mutant, which is defective for lipopolysaccharide out er core biosynthesis, was of intermediate virulence. Mutations in SPI-1 (hi lA and prgH) or aroA markedly reduced virulence and the severity of diarrhe a. Furthermore, histopathological examination of calves infected with SPI-1 or aroA mutants revealed a marked reduction or absence of intestinal lesio ns. These data suggest that virulence factors, such as SPI-1, which are req uired during intestinal colonization are more important for pathogenicity i n calves than are genes required during the systemic phase of S. typhimuriu m infection, including SPI-2 or the spy operon. This is in contrast to the degree of attenuation caused by these mutations in the mouse.