Increased expression of zif268 mRNA in rat retrosplenial cortex following administration of phencyclidine

Citation
Y. Shirayama et al., Increased expression of zif268 mRNA in rat retrosplenial cortex following administration of phencyclidine, BRAIN RES, 839(1), 1999, pp. 180-185
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
BRAIN RESEARCH
ISSN journal
0006-8993 → ACNP
Volume
839
Issue
1
Year of publication
1999
Pages
180 - 185
Database
ISI
SICI code
0006-8993(19990821)839:1<180:IEOZMI>2.0.ZU;2-Z
Abstract
Phencyclidine (PCP) has been shown to cause neurotoxicity in rat retrosplen ial cortex following a single administration, although the precise mechanis m underlying PCP-induced neurotoxicity is unclear. Using in situ hybridizat ion and immunohistochemistry, we studied the effects of PCP on expression o f immediate early gene zif268 mRNA and zif268 protein in the rat brain. Hig h constitutive levels of zif268 mRNA and zif268 immunoreactivity were obser ved in the brain of control rats. Administration of PCP (12.5, 25 or 50 mg/ kg, i.p., 6 h) caused marked induction of zif268 mRNA in the rat retrosplen ial cortex, in a dose-dependent manner. However, the basal levels of zif268 mRNA in the other regions of cerebral cortex were decreased by administrat ion of PCP. Emulsion-autoradiographical study suggested that marked express ion of zif268 mRNA was observed in the layers III and IV of retrosplenial c ortex where the neurotoxicity of PCP was detected. Furthermore, zif268 immu noreactivity in the layer IV of retrosplenial cortex was not changed by adm inistration of PCP (25 mg/kg, i.p., 5 h), but that in the other layers of r etrosplenial cortex was reduced by PCP. These results suggest that immediat e early gene zif268 may, in part, play a role in the neurotoxicity of NMDA receptor antagonists such as PCP. (C) 1999 Elsevier Science B.V. All rights reserved.