Activation of autologous or HLA-identical sibling cytotoxic T lymphocytes by blood derived dendritic cells pulsed with tumor cell extracts

Citation
Y. Osman et al., Activation of autologous or HLA-identical sibling cytotoxic T lymphocytes by blood derived dendritic cells pulsed with tumor cell extracts, ONCOL REP, 6(5), 1999, pp. 1057-1063
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology
Journal title
ONCOLOGY REPORTS
ISSN journal
1021-335X → ACNP
Volume
6
Issue
5
Year of publication
1999
Pages
1057 - 1063
Database
ISI
SICI code
1021-335X(199909/10)6:5<1057:AOAOHS>2.0.ZU;2-1
Abstract
This study describes the effect of dendritic cells (DC) pulsed with tumor c ell extracts on the induction and activation of autologous and HLA-identica l sibling tumor cell specific cytotoxic T lymphocytes (CTL). The subjects o f the study were patients with hematological malignancies (acute myeloid le ukemia, acute lymphoblastic leukemia, and multiple myeloma), and stem cell transplant donors. Although lymphocytes primed with non-pulsed DC did not s how definite cytotoxic activity against autologous or HLA-identical sibling patient tumor cells, both autologous and HLA-identical sibling CTL activat ed by DC pulsed with tumor cell extracts demonstrated markedly significant killing activity against tumor cells in the fashion of higher cytotoxicity with the increase of effector:target ratio (p<0.05). These findings reveal that DC pulsed with tumor cell extracts could be efficiently applicable in anti-tumor immunotherapy for patients with hematological malignancies.