Functionally heterogeneous CD8(+) T-cell memory is induced by Sendai virusinfection of mice

Citation
Ej. Usherwood et al., Functionally heterogeneous CD8(+) T-cell memory is induced by Sendai virusinfection of mice, J VIROLOGY, 73(9), 1999, pp. 7278-7286
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF VIROLOGY
ISSN journal
0022-538X → ACNP
Volume
73
Issue
9
Year of publication
1999
Pages
7278 - 7286
Database
ISI
SICI code
0022-538X(199909)73:9<7278:FHCTMI>2.0.ZU;2-M
Abstract
It has recently been established that memory CD8(+) T cells induced by vira l infection are maintained at unexpectedly high frequencies in the spleen. While it has been established that these memory cells are phenotypically he terogeneous, relatively little is known about the functional status of thes e cells. Here we investigated the proliferative potential of CD8(+) memory T cells induced by Sendai virus infection. High frequencies of CD8(+) T cel ls specific for both dominant and subdominant Sendai virus epitopes persist ed for many weeks after primary infection, and these cells were heterogeneo us with respect to CD62L expression (approximately 20% CD62L(hi) and 80% CD 62L(lo)). Reactivation of these cells with the antigenic peptide in vitro i nduced strong proliferation of antigen-specific CD8(+) T cells. However, ap proximately 20% of the cells failed to proliferate in vitro in response to a cognate peptide but nevertheless differentiated into effector cells and a cquired full cytotoxic potential. These cells also expressed high levels of CD62L (in marked contrast to the CD62L(lo) status of the proliferating cel ls in the culture). Direct isolation of CD62L(hi) and CD62L(lo) CD8(+) T ce lls from memory mice confirmed the correlation of this marker with prolifer ative potential. Taken together, these data demonstrate that Sendai virus i nfection induces high frequencies of memory CD8(+) T cells that are highly heterogeneous in terms of both their phenotype and their proliferative pote ntial.