Development of an analytical method for beryllium in airborne dust by micellar electrokinetic chromatography

Authors
Citation
M. Takaya, Development of an analytical method for beryllium in airborne dust by micellar electrokinetic chromatography, J CHROMAT A, 850(1-2), 1999, pp. 363-368
Citations number
9
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF CHROMATOGRAPHY A
ISSN journal
0021-9673 → ACNP
Volume
850
Issue
1-2
Year of publication
1999
Pages
363 - 368
Database
ISI
SICI code
0021-9673(19990730)850:1-2<363:DOAAMF>2.0.ZU;2-A
Abstract
The separation of diacetylacetonato-beryllium [Be(acac)(2)] from acetylacet one was achieved with micellar electrokinetic chromatography (MEKC) followe d by subsequent beryllium analysis carried out using capillary electrophore sis, Analysis using a separation and absorption detector, with a 50 mu m ca pillary cell, could detect approximately 1000 ppb of beryllium in the final sample. Be(acac)(2) is a promising reagent for absorption spectrophotometr y, because its molar absorption coefficient, (32 000 1 mol(-1) cm(-1)) is v ery large. As the complex and acetylacetone have a similar absorption wavel ength, the excess free ligand must removed from the sample to be measured. Acetylacetone is a weak acid with keto-enol tautomerism in aqueous solution . Acetylacetone and neutral Be(acac)(2) should be separated with capillary zone electrophoresis (CZE) using a neutral or basic buffer solution as the mobile phase. Although the pH and temperature of the mobile phase were opti mized, separation with CZE was interfered with by a portion of acetylaceton e. This interfering portion seems to be a neutral keto-form with the same m igration time as Be(acac)(2). As a neutral species separation method, MEKC with sodium dodecyl sulfate was tried and the separation was completed. The optimum pH value and buffer temperature are pH 7.8 and 15 degrees C, respe ctively. (C) 1999 Elsevier Science B.V. All rights reserved.