Stimulation of Golgi membrane tubulation and retrograde trafficking to theER by phospholipase A(2) activating protein (PLAP) peptide

Citation
Rs. Polizotto et al., Stimulation of Golgi membrane tubulation and retrograde trafficking to theER by phospholipase A(2) activating protein (PLAP) peptide, J CELL BIOC, 74(4), 1999, pp. 670-683
Citations number
42
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
JOURNAL OF CELLULAR BIOCHEMISTRY
ISSN journal
0730-2312 → ACNP
Volume
74
Issue
4
Year of publication
1999
Pages
670 - 683
Database
ISI
SICI code
0730-2312(19990915)74:4<670:SOGMTA>2.0.ZU;2-U
Abstract
Recent pharmacological studies using specific antagonists of phospholipase A(2) (PLA(2)) activity have suggested that the formation of Golgi membrane tubules, 60-80 nm in diameter and up to several microns long, both in vivo and in a cell-free cytosol-dependent reconstitution system, requires the ac tivity of a cytoplasmic Ca2+-independent PLA(2). We confirm and extend thes e studies by demonstrating that the stimulators of PLA(2), melittin and PLA (2) activating protein peptide (PLAPp), enhance cytosol-dependent Golgi mem brane tubulation. Starting with preparations of bovine brain cytosol (BBC), or a fraction of BBC that is highly enriched in tubulation activity, calle d the gel filtration (CF) fraction, that are at subsaturating concentration s for inducing tubulation in vitro, we found that increasing concentrations of melittin or PLAPp produced a linear and saturable stimulation of Golgi membrane tubulation. This stimulation was inhibited by cytosolic PLA(2) ant agonists, including the Ca2+-independent PLA(2)-specific antagonist, bromoe nol lactone. The stimulatory effect of PLAPp, and its inhibition by PLA(2) antagonists, was reproduced using a permeabilized cell system, which recons titutes both cytosol-dependent Golgi membrane tubulation and retrograde tra fficking to the endoplasmic reticulum (ER). Taken together, these results a re consistent with the idea that cytosolic PLA(2) activity is involved in t he formation of Golgi membrane tubules, which can serve as trafficking inte rmediates in Golgi-to-ER retrograde movement. (C) 1999 Wiley-Liss, Inc.