Glucokinase is concentrated in insulin-secretory granules of pancreatic B-cells

Citation
Y. Toyoda et al., Glucokinase is concentrated in insulin-secretory granules of pancreatic B-cells, HISTOCHEM C, 112(1), 1999, pp. 35-40
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
HISTOCHEMISTRY AND CELL BIOLOGY
ISSN journal
0948-6143 → ACNP
Volume
112
Issue
1
Year of publication
1999
Pages
35 - 40
Database
ISI
SICI code
0948-6143(199907)112:1<35:GICIIG>2.0.ZU;2-Q
Abstract
We immunohistochemically examined the distribution of glucokinase (GK) in t he B-cells of pancreatic islets of normal rats. GK was stained punctately i n the cytoplasm of B-cells when examined under the light microscope. By use of a double-immunostaining technique, most of the GK immunoreactivity was observed to bie colocalized with insulin immunoreactivity. Electron microsc opic examination by the immunogold method revealed that GK immunoreactivity was predominantly located within insulin-secretory granules of pancreatic B-cells.