D-Altritol nucleic acids (ANA): Hybridisation properties, stability, and initial structural analysis

Citation
B. Allart et al., D-Altritol nucleic acids (ANA): Hybridisation properties, stability, and initial structural analysis, CHEM-EUR J, 5(8), 1999, pp. 2424-2431
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry
Journal title
CHEMISTRY-A EUROPEAN JOURNAL
ISSN journal
0947-6539 → ACNP
Volume
5
Issue
8
Year of publication
1999
Pages
2424 - 2431
Database
ISI
SICI code
0947-6539(199906)5:8<2424:DNA(HP>2.0.ZU;2-G
Abstract
Oligonucleotides composed of a phosphorylated D-altritol backbone with nucl eobases introduced in the 2'-position, hybridise strongly and sequence-sele ctively with RNA in an antiparallel way. The order of hybridisation strengt h is: dsANA > ANA:RNA > ANA:DNA. Complexes between ANA and RNA dr DNA are m ore stable than between HNA and natural oligonucleotides. The dsANA hybrid is extremely stable. When compared with an identical dsDNA sequence, the De lta T-m per modification is +10.2 degrees C for a hexamer. CD spectral anal ysis indicates that ANA complexes are very similar to the A-form dsRNA dupl ex. ANA are stable in alkaline medium up to pH 12 and, likewise, are not de graded in human serum.