High-performance liquid chromatography study of the pharmacokinetics of various spin traps for application to in vivo spin trapping

Citation
Kj. Liu et al., High-performance liquid chromatography study of the pharmacokinetics of various spin traps for application to in vivo spin trapping, FREE RAD B, 27(1-2), 1999, pp. 82-89
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FREE RADICAL BIOLOGY AND MEDICINE
ISSN journal
0891-5849 → ACNP
Volume
27
Issue
1-2
Year of publication
1999
Pages
82 - 89
Database
ISI
SICI code
0891-5849(199907)27:1-2<82:HLCSOT>2.0.ZU;2-C
Abstract
In vivo spin trapping is potentially a very useful tool to investigate the role of free radicals in physiologic processes and disease development. Unf ortunately, knowledge on the stability and distribution of spin traps in li ving systems is limited. Therefore, in our study, we selected 11 acyclic an d cyclic nitrone spin traps with diverse properties to determine their phar macokinetics in mice. At varying times after intraperitoneal administration , we measured the concentration of the spin traps in the liver, heart, and blood. Our results showed that most spin traps were rapidly absorbed and we re approximately evenly distributed throughout the mouse body. It was also found that most of the traps were relatively stable in vivo with more than half of the injected amount still available for spin trapping free radicals after an hour. Two of the 11 tested spin traps, however, decomposed after injection. These results indicate that for a successful in vivo spin trappi ng experiment, the stability of the spin trap is not of major concern, but the time course of distribution may be important. (C) 1999 Elsevier Science Inc.