Synergistic action of apoptosis induced by eicosapentaenoic acid and TNP-470 on human breast cancer cells

Citation
D. Yamamoto et al., Synergistic action of apoptosis induced by eicosapentaenoic acid and TNP-470 on human breast cancer cells, BREAST CANC, 55(2), 1999, pp. 149-160
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology,"Onconogenesis & Cancer Research
Journal title
BREAST CANCER RESEARCH AND TREATMENT
ISSN journal
0167-6806 → ACNP
Volume
55
Issue
2
Year of publication
1999
Pages
149 - 160
Database
ISI
SICI code
0167-6806(199905)55:2<149:SAOAIB>2.0.ZU;2-D
Abstract
The effects of eicosapentaenoic acid (EPA) and an angiogenesis inhibitor (T NP-470) on the suppression of breast cancer cell growth were examined in fi ve human breast cancer cell lines (MDA-MB-231, T-47D, MCF-7, KPL-1, and MKL -F). In all five cell lines, EPA and TNP-470 alone both showed tumor growth inhibition in a time- and dose-dependent manner, and in combination, a syn ergistic effect was seen at high concentrations. EPA plus TNP-470 treatment evoked apoptosis as confirmed by the appearance of sub G1 populations, by DNA fragmentation, and by cell morphology. With the combination, the expres sion of Bax and Bc1-x(S), the apoptosis-enhancing proteins, was more up-reg ulated and that of Bcl-2 and Bcl-x(L), the apoptosis-suppressing proteins, was more down-regulated compared to the use of EPA or TNP-470 alone, sugges ting that their synergistic effect was due to an acceleration of apoptosis.