Purpose: To test the anti-inflammatory effect of propolis, a natural bee-pr
oduced compound, and compare it with corticosteroids fur the: treatment of
endotoxin-induced uveitis (EIU).
Methods: EIU was produced in all rabbits by unilateral intravitreal injecti
on of 2,000 ng Salmonella typhimurium endotoxin. The animals were then divi
ded randomly into three groups as follows: group A received no treatment (c
ontrol), group B received methylprednisolone (5 mg/0.1 mL) (positive contro
l); and group C received propolis (5 mg/0.16 mL) by anterior sub-Tenon inje
ction at the time of uveitis induction and at 4 and 8 hours after induction
. Inflammation was evaluated by clinical manifestations and by measuring th
e protein concentration and inflammatory cell content of the aqueous humor.
Results: The clinical grade, cell count: and protein levels in the aqueous
humor were: control group (6.0 +/- 0.8, 2,519 +/- 470 cells/mu L. 32.9 +/-
2.4 mg/mL); methylprednisolone group (1.8 +/- 0.7, 572 +/- 137 cells/mu L.
15.2 +/- 1.8 mg/mL); and propolis group (2.3 +/- 0.5, 503 +/- 124 cells/mu
L, 13.8 +/- 1.5 mg/mL). Statistically significant differences were recorded
in the treatment groups when compared to the control group (P < .001). The
effects of methylprednisolone and propolIs on EIU were similar (P > .05).
Conclusions: Propolis showed significant anti-inflammatory effects on EIU i
ii rabbits. The mechanism of its action warrants further investigation. Jpn
J Ophthalmol 1999;43:285-289. (C) 1999 Japanese Ophthalmological Society.