Effects of angiotensin I-converting enzyme inhibitor from Ashitaba (Angelica keiskei) on blood pressure of spontaneously hypertensive rats

Citation
E. Shimizu et al., Effects of angiotensin I-converting enzyme inhibitor from Ashitaba (Angelica keiskei) on blood pressure of spontaneously hypertensive rats, J NUTR SC V, 45(3), 1999, pp. 375-383
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Food Science/Nutrition","Endocrinology, Nutrition & Metabolism
Journal title
JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY
ISSN journal
0301-4800 → ACNP
Volume
45
Issue
3
Year of publication
1999
Pages
375 - 383
Database
ISI
SICI code
0301-4800(199906)45:3<375:EOAIEI>2.0.ZU;2-H
Abstract
The inhibitory activity of angiotensin I-converting enzyme (ACE) was extrac ted with 80% ethanol from the leaves of Ashitaba (Angelica keiskei). The pr esent ACE inhibitor was fractionated and separated with various chromatogra phies. The antihypertensive effects of the sample (G fraction) from Ashitab a on spontaneously hypertensive rats (SHR) were observed by long-term admin istration for 10 wk. Another sample (S fraction) from Ashitaba also had ant ihypertensive effects after a single intravenous administration to SHR. The sample was further purified by using several chromatographies. The ACE inh ibitor fraction was characterized as follows: no significant absorbance, a zwitterion, a water-soluble substance and a positive ninhydrin reaction. Ac cording to a mass spectrum analysis, the molecular weight of the ACE inhibi tor was determined to be 303 and Na-salt ions of carboxyl groups were detec ted. The ACE inhibitor from Ashitaba contained in the anti-hypertensive fra ction was speculated to be very similar to authentic nicotianamine based on a comparative study of inhibitory activity, mass spectrum analysis and thi n-layer chromatographies.