Nitric oxide inhibits HIV Tat-induced NF-kappa B activation

Citation
F. Chen et al., Nitric oxide inhibits HIV Tat-induced NF-kappa B activation, AM J PATH, 155(1), 1999, pp. 275-284
Citations number
56
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Journal title
AMERICAN JOURNAL OF PATHOLOGY
ISSN journal
0002-9440 → ACNP
Volume
155
Issue
1
Year of publication
1999
Pages
275 - 284
Database
ISI
SICI code
0002-9440(199907)155:1<275:NOIHTN>2.0.ZU;2-6
Abstract
To evaluate the roles of nitric oxide (NO) on human immunodeficiency virus (HIV) Tat-induced transactivation of HIV long terminal repeat (HIV-LTR), we examined the effect of NO in the regulation of nuclear factor (NF)-kappa B , a key transcription factor involved in HIV gene expression and viral repl ication. In the present study, we demonstrate that HIV Tat activates NF-kap pa B and that this activation can be attenuated by endogenous or exogenous NO. Inhibition of endogenous NO production with the NO synthase (NOS) inhib itor L-NMMA causes a significant increase in Tat-induced NF-kappa B activit y. In addition, NO attenuates signal-initiated degradation of I kappa B alp ha, an intracellular inhibitor of NF-kappa B, and blocks the DNA binding ac tivity of the NF-kappa B p50/p50 homodimer and p50/p65 heterodimer. To dete rmine how NO is induced by HIV Tat, reverse transcription polymerase chain reaction was used to demonstrate the induction of NOS-2 and NOS-3 mRNA by T at. Although a putative NF-kappa B binding site was identified in the -74 G GAGAGCCCCC -64 region of the NOS-3 gene promoter, gel mobility shift assays and site-directed mutation analyses suggest that the putative NF-kappa B s ite is not of primary importance. Rather, several Sp-1 sites adjoining the putative NF-kappa B binding site in the promoter region of NOS-3 gene are r equired for the induction of NOS-3 gene expression by Tat.