Immunohistochemical evidence for the brevican-tenascin-R interaction: Colocalization in perineuronal nets suggests a physiological role for the interaction in the adult rat brain

Citation
K. Hagihara et al., Immunohistochemical evidence for the brevican-tenascin-R interaction: Colocalization in perineuronal nets suggests a physiological role for the interaction in the adult rat brain, J COMP NEUR, 410(2), 1999, pp. 256-264
Citations number
44
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
JOURNAL OF COMPARATIVE NEUROLOGY
ISSN journal
0021-9967 → ACNP
Volume
410
Issue
2
Year of publication
1999
Pages
256 - 264
Database
ISI
SICI code
0021-9967(19990726)410:2<256:IEFTBI>2.0.ZU;2-4
Abstract
Brevican is one of the most abundant chondroitin sulfate proteoglycans in t he adult rat brain. We have recently shown that the C-type lectin domain of brevican binds fibronectin type III domains 3-5 of tenascin-R. Here we rep ort strong evidence for a physiological basis for this interaction. Substan tial brevican immunoreactivity was detected in a number of nuclei and in th e reticular formations throughout the midbrain and hindbrain, including, bu t not limited to, the deep cerebellar nuclei, the trapezoid body, the red n ucleus, the oculomotor nucleus, the vestibular nucleus, the cochlear nucleu s, the gigantocellular reticular nucleus, the motor trigeminal nucleus, and the lateral superior olive. Most of the brevican immunoreactivity exhibite d pericellular and reticular staining patterns. In almost all of these site s, brevican immunoreactivity colocalized with that of tenascin-R, which was also substantially codistributed with versican, another member of the lect ican family. Detailed analysis revealed that the pericellular staining of b revican resembled that in perineuronal nets in which tenascin-R has been lo calized. Immunoelectron microscopy identified brevican immunoreactivity in the intercellular spaces surrounding presynaptic boutons and on their surfa ces, but not in the synaptic clefts or in their immediate vicinity, a distr ibution pattern consistent with perineuronal nets. Taken together, our resu lts provide strong evidence that the previously reported interactions betwe en brevican and tenascin-R may play a functional role within the perineuron al nets. (C) 1999 Wiley-Liss, Inc.